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EneCats are enzyme biocatalysts capable of catalysing asymmetric activated alkene reduction (C=C to CH-CH). They are active against a variety of alkenes containing ketones, aldehydes, nitro, dicarboxylates, diesters as well as malemides, steroids, nitroaromatics and ketoisophorone. These robust biocatalysts work in conjunction with NAD(P)H, or cofactor recycling systems, under mild conditions to generate single enantiomeric products in many cases. This collection of biocatalysts can be used to screen for activity against your chosen synthon(s). It includes a thermostable enzyme and a non-flavin dependent biocatalyst.

Wild Type Enzymes

Wild type PETNR (pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2).

Wild type thermophilic Old Yellow Enzyme (TOYE from Thermoanaerobacter pseudethanolicus E39).

Wild type NtDBR (non-OYE ene-reductase from Nicotiana tabacum).

 

Individual enzymes are provided as sequence verified genes in an expression vector as glycerol stocks within an expression strain (1.0 mL).

Biocatalyst Enzymes: Wild-type

Wild type PETNR, TOYE, NtDBR biocatalyst enzymes

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Click2Go Annual Research Licence Materials 1.00

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Further details
Term: 12 months
Seats: n/a

Price excl. VAT: £500.00

Click2Go Annual Commercial Licence Materials 1.00

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Further details
Term: 12 months
Seats: n/a

Price excl. VAT: £1000.00